用智能手机也可以检测新冠病毒了手机有新冠病毒吗( 五 ) _小知识

have been 来过
necessary for 对…是必要的
that is 即 ; 用于纠正之前说过的内容
multiplex 多路复用 ; 多路传输 ; 多重发讯 ; 多厅影院 ; 多银幕电影院 ; 多样的 ; 多路传输的 ; 多路复用的 ; 多元的
supply chain 供应链
isothermal 等温的；等温线的 ; 等温线
reagents 试剂 ; reagent的复数
saliva 唾液
is subject to 受支配，从属于 ; 有…倾向的
POC 停靠港（port of call） ; 颗粒有机碳 ; 手机对讲 ; 概念证明 ; 颗粒态有机碳
Smartphones are ideally suited to meet the need for low-cost, widely accessible clinical POC diagnostic tools, with smartphone global use estimated at nearly half the world’s population.28 The potential to transform mobile phones into clinical POC diagnostic tools is evidenced by a number of detection modalities, including those based on optical and fluorescence imaging, microfluidic chips, biosensors, microelectronics, lateral flow, and nucleic acid or immunologic detection.29-40 However, these innovative systems generally require the coupling of the mobile phone to specialized accessory diagnostic devices, custom reagents, or sophisticated protocols. Examples include a LAMP system that uses a 3-D cartridge and a smartphone-based reader37; a centrifugal microfluidic platform with a smartphone read-out39; and a CRISPR-Cas13a assay that features mobile phone microscopy.38
In this study, we investigate a low-tech test, termed smartphone-based real-time loop-mediated isothermal amplification (smaRT-LAMP), to see whether it possesses the combined attributes of speed, sensitivity, low cost, scalability, and accessibility for rapid and frequent POC testing.

智能手机非常适合满足对低成本、广泛使用的临床 POC 诊断工具的需求，智能手机的全球使用率估计占世界人口的近一半。28将手机转变为临床 POC 诊断工具的潜力已通过多种检测方式得到证明，包括基于光学和荧光成像的检测方式、微流控芯片、生物传感器、微电子学、横向流动以及核酸或免疫检测。29 - 40然而，这些创新系统通常需要将移动电话与专门的辅助诊断设备、定制试剂或复杂协议相结合。示例包括使用 3-D 墨盒的 LAMP 系统和基于智能手机的阅读器37; 带有智能手机读数的离心微流体平台39；以及以手机显微镜为特色的 CRISPR-Cas13a 分析。38
在这项研究中，我们调查了一项低技术测试，称为基于智能手机的实时环路介导等温放大 (smaRT-LAMP)，以了解它是否具有速度、灵敏度、低成本、可扩展性和可访问性等综合属性用于快速和频繁的 POC 测试。
mobile phones 移动电话 ; 手机 ; mobile phone的复数
a number of 一些 ; 许多的
based on 以……为基础；在……基础上，以……为依据
nucleic acid 核酸
immunologic 免疫学的low-tech 不涉及最现代技术的
isothermal 等温的；等温线的 ; 等温线
amplification 扩大；膨胀
possesses 有 ; 拥有 ; 具有 ; 攫住 ; 支配 ; 控制 ; possess的第三人称单数
sensitivity 体贴 ; 体恤 ; 体察 ; 敏锐的感觉 ; 悟性 ; 容易生气 ; 易被惹恼 ; 敏感 ; 敏感性 ; 灵敏度
low cost 低成本；廉价
scalability 可扩展性；可伸缩性；可量测性
frequent 频繁的 ; 经常发生的 ; 常去，常到
POC 停靠港（port of call） ; 颗粒有机碳 ; 手机对讲 ; 概念证明 ; 颗粒态有机碳

Methods

方法

Human participant approval was obtained from the Institutional Human Subjects Use Committee of the University of California, Santa Barbara, and the institutional review board of Santa Barbara Cottage Hospital. Written informed consent was obtained from all participants. An in-person or remote video interpreter was used as needed. This cohort study followed the Standards for Reporting of Diagnostic Accuracy (STARD) reporting guideline. Race and ethnicity were not considered in this study.

人类参与者的批准是从加州大学圣巴巴拉分校的机构人类受试者使用委员会和圣巴巴拉小屋医院的机构审查委员会获得的。所有参与者都获得了书面知情同意书。根据需要使用现场或远程视频口译员。该队列研究遵循诊断准确性报告标准 ( STARD ) 报告指南。本研究未考虑种族和民族。
University of California 加利福尼亚大学
review board 评审委员会;评审组
Cottage Hospital 乡村小医院 ; 乡间诊疗所
informed consent 知情同意，知会同意
cohort study 队列研究法

LAMP Protocol Advances That Increase Sensitivity and Eliminate False Positives Due to Primer-Dimer Amplification

LAMP 协议的进步提高了灵敏度并消除了由于引物二聚体扩增导致的误报

The clinical utility of LAMP-based diagnostics have been limited by false positives (primer-dimer amplification).14-16 This technical challenge was overcome by the development of a LAMP protocol that optimizes experimental conditions for viral RNA stability and cDNA synthesis, which both increased sensitivity and effectively eliminated false positives due to primer-dimer amplification (eMethods, eTable 1, eFigure 1, and eFigure 2 in the Supplement). We found that in addition to reaction mixture composition, the order of assembly of the reaction mixture components was critical to improve LAMP performance and reduce primer-dimer formation. Furthermore, the smartphone app imparts a 25-minute reaction time cutoff to distinguish specimen-sample amplification (early) from primer-dimer amplification (late).

用智能手机也可以检测新冠病毒了 手机有新冠病毒吗( 五 )

用智能手机也可以检测新冠病毒了手机有新冠病毒吗( 五 )