1、化瘀通络汤对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究目 录摘要I-1前言12材料与仪器22.1实验动物22.2实验药物22.3实验试剂32.4实验仪器33方法33.1实验动物及其分组33.2MCAO模型的建立33.3神经功能缺损评分43.4脑梗死体积的测定43.5脑组织病理制片的观察43.6大鼠腹主动脉采血测血清中IL-6的含量53.7统计学方法54结果54.1神经缺损评分及脑梗死体积54.2脑组织病理结果64.3血清中IL-6的含量75讨论85.1 实验动物的选择85.2 实验模型的选择85.3实验分组及阳性对照药的选择85.4测血清中IL-6对大鼠的影响95.5处方分析9参考文献10综 。

2、述11致谢17化瘀通络汤对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究摘要:目的 研究化瘀通络汤如何减轻局灶性脑缺血再灌注大鼠的炎症反应 ， 从而减轻脑缺血再灌注损伤 ， 起到保护作用 。
方法 120只SD大鼠随机分为假手术组、模型对照组、化瘀通络汤低剂量组、中剂量组、高剂量组、阳性对照组（步长脑心通） ， 改良线栓法建立MCAO模型 ， TTC染色测定脑梗死体积 ， HE染色观察脑组织病理结构 ， ELISA法测定大鼠血清中IL-6的含量 。
结果 与模型对照组比较 ， 各给药组大鼠神经功能评分明显偏低 ， 结果有统计学意义（P0.05）；与模型对照组比较 ， 化瘀通络汤高剂量组、阳性对照组大鼠脑梗死体积明显减小 ， 结果有统计学意义（ 。

3、P0.05） 。
与假手术组相比,模型对照组大鼠脑部海马区呈现明显的水肿情况 ， 出现神经元细胞大量坏死、变性 。
给药组与模型对照组相比 ， 正常神经元数量增多且排列规则 ， 脑组织变性、坏死的范围显著下降 。
与模型对照组比较 ， 各给药组大鼠血清中IL-6的含量明显降低 ， 结果有统计意义（P0.05）；与阳性对照组比较 ， 化瘀通络汤低剂量组、高剂量组大鼠中IL-6的含量相对较低 ， 化瘀通络汤高剂量组的治疗效果更好 ， 结果有统计意义（P0.05） 。
结论 化瘀通络汤能显著降低神经功能缺损评分 ， 减少脑梗死体积 ， 促进神经元生长 ， 减少脑组织变性和坏死 ， 降低血清中IL-6的含量 ， 对脑缺血再灌注损伤大鼠有很好的保护作用 。
关键词：化瘀通络 。

4、汤、MCAO模型、IL-6、脑梗死Experimental study of Huayu Tongluo decoction against cerebral ischemia reperfusion injury of rat Abstract: Objective To study how Huayu tongluo decoction can reduce the inflammatory response of focal cerebral ischemia-reperfusion rats, thus alleviating cerebral ischemia-reperfusion 。

5、 injury. Methods 120 SD Rats were randomly divided into sham operation Group, model control group, Huayu tongluo decoction low dosage Group, medium dosage Group, high dosage Group, Positive control group (step Brain Pass), modified line suppository method to establish Mcao model, TTC staining to det 。

6、ermine cerebral infarction volume, he staining to observe brain tissue pathological structure, The content of IL-6 in rat serum was determined by Elisa. Results compared with the model control group, the neurological function score of each drug group was significantly low, the result was statistical 。

7、ly significant (p0.05), compared with the model control group, the volume of cerebral infarction in the high dose group and the positive control group decreased obviously, and the results were statistically significant (p0.05). Compared with sham operation Group, the hippocampus of rats in model con 。

8、trol group showed obvious edema, and neuronal cells were degeneration. Compared with the model control group, the number of normal neurons increased and the range of brain tissue degeneration and necrosis decreased significantly. Compared with the model control group, the content of IL-6 in the seru 。

9、m of rats in each drug group decreased significantly, the results were statistically significant (p0.05), compared with the positive control group, the content of IL-6 in the low dosage group and high dose group was relatively low, and the therapeutic effect of Huayu Tongluo decoction in high dosage 。

10、 group was better, the results were statistically significant ( p0.05). Conclusion Huayu Tongluo decoction can significantly reduce the score of neurologic impairment, reduce the volume of cerebral infarction, promote the growth of neurons, reduce the degeneration and necrosis of brain tissue, reduc 。

11、e the content of IL-6 in serum, and have a good protective effect on rats with cerebral ischemia-reperfusion injury. Keywords: Huayu Tongluo decoction;
the MCAO model;
IL-6;
the brain stem dieIII1 前言脑梗塞因其发病率高、致残率高、死亡率高、复发率高的特点 ， 已成为严重影响社会和家庭的疾病之一 。

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标题：化瘀通络汤对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究|化瘀通络汤对大鼠局灶性脑缺血再灌注损伤保护作用的实验研究中药学专业